Abstract & Overview
Biofilm bacteria pose a profound threat to human and animal health. This study helps to explore the potential anti-gene expression effect of the green copper nanoparticles (CuONPs) on biofilm genes in strong biofilm forming E. coli The anti- gene expression of green synthesis of CuONPs in different concentrations (MIC and Sub MIC) were evaluated against E. coli biofilm genes (luxS, mqsR, fliA) with housekeeping GapA gene by Real- time quantitive polymerase chain reaction (RT- qPCR) after treatment in comparative with control. The results revealed that green CuONPs significant effective on biofilm gene expression by down regulate of luxS gene in fold change (1.2, 4.92) in human E. coli isolates and (0.744, 6.01) in animals in MIC and Sub MIC concentration respectively, with comparative control (14.870) and (12.616) respectively. The expression of Mqsr gene was down regulate after treatment with green CuONPs fold change (0.95, 9.06) and (1.078, 6.7) in human and animals in MIC and Sub MIC concentrations respectively with comparative control (13.9) and (18.8) respectively. Also, the expression of Flia gene was decrease after treatment with green CuONPs fold change (1.054, 7.06) and (0.5, 7.4) in human and animals of MIC and Sub MIC Concentration respectively with comparative control (16.318) and (20.353) respectively Conclusion: Green CuONPs showed an excellent inhibitory effect and anti-biofilm activity against MDR biofilm E. coli genetically which act as down- regulator of biofilm genes in strong biofilm forming E.coli.