Abstract & Overview
This study conducted tests on various viruses with ash water, which can be obtained very simply. The study aims to produce a liquid that can block viruses and be obtained from waste combustion products, unlike disinfectants that have a place in daily life. The present study tested ash water containing methyl stearate and methyl esters, octadecenoic, octadecanoic acid, and methyl esters and their derivatives against DNA- and RNA-based viruses. Bovine Rotavirus, Bovine Coronavirus, and Parainfluenza virus-3 were used as RNA-based viruses, and Bovine Adenovirus and Bovine Herpesvirus were used as DNA-based viruses. MTT tests were conducted in the in-vitro cytotoxicity analysis of healthy cells. After the MTT tests, ash water was filtered and adjusted to pH 7.4. It was found that, when diluted at the rate of 1/32, ash water showed no harmful effect on healthy cells. In the tests against Bovine Parainfluenza virus, the compound showed no activity. In the first 24 hours, the compound was found to block the Bovine Coronavirus, one of the RNA-based viruses, by 100% at 0.01 MOI (10-6) concentration and by 50% at 0.1 MOI (10-5) concentration. In the first 24 hours, the compound was found to block Bovine Rotavirus by 100% at 0.1 and 0.01 MOI (10-4 and 10-5) concentrations and by 50% at 1 MOI (10-3) concentrations. The compound showed no activity in the test conducted against Bovine Herpesvirus, one of the DNA-based viruses. When tested against Bovine Adenovirus, the compound blocked the virus by 50% in the first 24 hours at 0.01 MOI (10-3) concentration. As a result, it has been observed that ash water, which can be easily produced instead of disinfectants containing chemicals, is effective against two viruses. It has been understood that if the studies on ash water are improved, a natural liquid effective on viruses can be developed.